اثر پرایمینگ و فرسودگی بر شاخص‌های رشد و فعالیت آنزیم‌های آنتی‌اکسیدان در بذر ذرت هیبرید سینگل کراس 704

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری دانشگاه محقق اردبیلی

2 گروه زراعت و اصلاح نباتات دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی

چکیده

به‌منظور بررسی اثر پرایمینگ و فرسودگی بر شاخص‌های رشد و فعالیت آنزیم‌های آنتی‌اکسیدان در بذر ذرت هیبرید سینگل کراس 704 آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در دانشگاه محقق اردبیلی در سال 1401 اجرا شد. تیمارهای آزمایشی شامل تیمار پیری تسریع شده در سه سطح (0، 7 و 14 روز) و چهار سطح پرایمینگ (شاهد، هیدروپرایمینگ، جیبرلین (20 میلی‌گرم در لیتر) و اسید سالیسیلیک (100 میلی‌گرم در لیتر) بود. نتایج نشان داد که فرسودگی شاخص‌های رشد شامل طول ریشه‌چه، طول ساقه‌چه، طول گیاهچه، وزن تر ریشه‌چه و ساقه‌چه، وزن خشک ریشه‌چه و ساقه‌چه، شاخص طولی و وزنی بنیه بذر را کاهش داد، ولی انواع پرایمینگ به‌ویژه پرایمینگ با جیبرلین این صفت‌ها را بهبود داد. میزان فعالیت آنزیم‌های کاتالاز، سوپراکسید دیسمیوتاز و گلوتاتیون ردوکتاز در تیمار جیبرلین و فرسودگی 14 روز در مقایسه با شاهد به ترتیب در حدود 73%، 63% و 21% افزایش نشان داد. بیش‌ترین میزان فعالیت آنزیم پراکسیداز (797/1 واحد بر میلی گرم پروتئین) در پرایمینگ با جیبرلین مشاهده شد. در مجموع، کاربرد پیش‌تیمار جیبرلین موجب تقویت شاخص‌های رشد و فعالیت آنزیم‌های آنتی‌اکسیدان بذرهای ضعیف ذرت شد و می‌توان جهت افزایش بنیه بذرهای ضعیف از این تیمار استفاده کرد.

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