عنوان: اثر نانو‌سریم (CeO2) و نیتروپروساید سدیم (SNP) بر جوانه‌زنی و رشد گیاهچه گندم رقم پیشگام تحت تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 عضو هیات علمی گروه زراعت و اصلاح نباتات دانشگاه بوعلی سینا

2 گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه بوعلی سینا همدان

چکیده

به منظور ارزیابی تاثیر نانو‌ذره سریم و نیتروپروساید سدیم بر شاخص‌های جوانه‌زنی ، بیوشیمیایی و رشد گندم تحت تنش خشکی، آزمایشی به صورت فاکتوریل در قالب طرح کامل تصادفی با سه تکرار انجام شد. اثرات نانوذره ‌سریم در غلظت‌های 0، 500، 1500 و 2000 میلی‌گرم در لیتر و نیتروپروساید سدیم به عنوان آزاد‌کننده اکسید نیتریک در غلظت‌های 0 و 100 میکرومولار تحت سطوح تنش خشکی 0، 4/0- و 8/0- مگاپاسکال مورد بررسی قرار گرفت. تنش خشکی موجب کاهش معنی‌دار اکثر صفات مرتبط با جوانه‌زنی از جمله درصد و سرعت جوانه‌زنی گردید، همچنین خشکی باعث افزایش میانگین زمان جوانه‌زنی، فعالیت آنزیم‌های سوپر‌اکسید دیسموتاز، کاتالاز، آسکوربات پراکسیداز و محتوی مالون دی‌آلدهید در گیاهچه‌های گندم شد. کاربرد همزمان نانوذره ‌سریم و نیتروپروساید سدیم بر اغلب صفات مورد مطالعه تاثیر معنی‌داری داشت. کاربرد نانو‌سریم (1000 میلی‌گرم در لیتر) به همراه نیتروپروساید سدیم درصد جوانه‌زنی و شاخص بنیه بذر را به ترتیب 5/44 و 5/75 درصد تحت تنش خشکی 8/0- مگاپاسکال افزایش داد. تیمار مذکور تحت تنش خشکی 8/0- مگاپاسکال، فعالیت آنزیم‌های سوپر‌اکسید دیسموتاز و آسکوربات پراکسیداز را به ترتیب 7/19 و 1/41 درصد افزایش و میزان مالون ‌دی‌آلدهید گیاهچه گندم را 5/33 درصد کاهش داد. به نظر می‌رسد نیتروپروساید سدیم و همچنین نانو‌سریم از طریق بهبود شاخص‌های جوانه‌زنی و تقویت سیستم دفاع آنتی‌اکسیدانی موجب کاهش اثرات مضر تنش خشکی بر گیاهچه‌ گندم می‌شود.

کلیدواژه‌ها


Abdi, H., M. Bihamta, E. Aziz Ov, and R. Chogan. 2015. Investigation effect of drought stress level of PEG 6000 on seed germination principle and its relation with drought tolerance index in promising lines and cultivares of bread wheat (Triticum. aestivum L.). Iran. J. FieldCrops Res. 12: 582–596. (In Persian, with English Abstract)
Aebi, H. 1984. Catalase in vitro. Methods Enzymol. 105: 121–126.
Alessandro, M., P. Filip, F. Guido, Z. Costanza, F. Marta, P. Barbara, V. Massimo, M. Fabiano, M. Rita, and M. Luca. 2016. Engineered nanomaterials and crops: physiology and growth of barley as affected by nanoscale cerium oxide. Ital. J. Agron.  11: 149–157.
Amooaghaie, R., F. TabaTabaei, A.M. Ahadi. 2015. Role of hematin and sodium nitroprusside in regulating Brassica nigra seed germination under nano silver and nitrate silver stresses. Eco. Environ. Safe. 113: 259–270.
Anandkumar, M., C.H. Ramamurthy, C. Thirunavukkarasu, and K. Suresh Babu. 2015. Influence of age on the free-radical scavenging ability of CeO2 and Au/CeO2 nanoparticles. J. Mater. Sci. 50: 2522–2531
Andersen, C.P., G. King, M. Plocher, M. Storm, L.R. Pokhrel, M.G. Johnson, P.T. Rygiewicz. 2016. Germination and early plant development of ten plant species exposed to titanium dioxide and cerium oxide nanoparticles. Environ. Toxico. Chem. 35: 222–2229.
Bajji, M., J.M. Kinet, and S. Lutts. 2002. Osmotic and ionic effects of NaCl on germination, early seedling growth, and ion content of Atriplex halimus (Chenopodiaceae). Can. J. Bot. 80: 297–304.
Białecka, B., and J. Kępczyński. 2010. Germination, α-, β-amylase and total dehydrogenase activities of Amaranthus caudatus seeds under water stress In the presence of ethephon or gibberellin A3. Acta Biol. Cracov. Ser. Bot. 52: 7–12.
Corral-Diaz B., J.R. Peralta-Videa, E. Alvarez-Parrilla, J. Rodrigo-García, P. Osuna-Avila, G.H. Niu, J.A. Hernandez-Viezcas, and J.L. Gardea-Torresdey. 2014. Cerium oxide nanoparticles alter the antioxidant capacity but do not impact tuber ionome in Raphanus sativus (L). Plant Physiol Biochem 84: 277–285.
Ellis, R.H., and E.H. Roberts. 1981. The quantification of ageing and survival in orthodox seeds. Seed Sci. Technol. 9: 377–409.
Giannopolitis, C.N., and S.K. Ries. 1977. Superoxide dismutases: I. occurrence in higher plants. Plant Physiol. 59: 309–314.
Heath, R.L. and L. Pacher. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometery of fatty acid peroxidation. Arch. Biochem. Biophys. 125: 189–198. 
Khan, M.A., and I.A. Ungar. 1984. The effect of salinity stress and temperature on the germination of polymorphic seeds and growth of atriplex triangularis willd. Amer. J. Bot. 71: 481–489.
Khodakovskaya, M.V., K. de Silva, A.S. Biris, E. Dervishi, and H. Villagarcia. 2012. Carbon nanotubes induce growth enhancement of tobacco cells. ACS Nano. 6: 2128–35.
Khot, L.R., S. Sankaran, J.M. Maja, R. Ehsani, and E.W. Schuster. 2012. Applications of nanomaterials in agricultural production and crop protection: a review. Crop Prot. 35: 64–70.
Krasylenko, Y.A., A.I. Yemets, and Y.B. Blume. 2010. Functional role of nitric oxide in plants. Russ. J. Plant Physiol. 57: 451.
Liu, D., X. Wang, , Y. Lin, Z. Chen, H. Xu, and L. Wang. 2012. The effects of cerium on the growth and some antioxidant metabolisms in rice seedlings. Environ. Sci. Pollut. Res. 19: 3282–3291.
Liu, H.Y., X. Yu, D.Y. Cui, M.H. Sun, W.N. Sun, Z.C. Tang, S.S. Kwak, and W.A. Su. 2007. The role of water channel proteins and nitric oxide signaling in rice seed germination. Cell Res. 17: 638–649.
López-Moreno, M.L., G. de La Rosar, J.A. Hernández-Viezcas, J.R. Peralta-Videa, and J.L. Gardea-Torresdey. 2010. X-ray absorption spectroscopy (XAS) corroboration of the uptake and storage of CeO2 nanoparticles and assessment of their differential toxicity in four edible plant species. J. Agric. Food Chem. 58: 3689-3693.
Lu, Y., X. Li, M. He, X. Zhao, Y. Liu, Y. Cui, Y. Pan, and H. Tan. 2010. Seedlings growth and antioxidative enzyme activities in leaves under heavy metal stress differ between two desert plants: A perennial (Peganum harmala) and an annual (Halogeton glomeratus) grass. Acta Physiol. Plant. 32: 583−590.
Majumdar, S., J.R. Peralta-Videa, S. Bandyopadhyay, H. Castillo-Michel, J.A. Hernandez, S. Sahi, and J.L. Gardea-Torresdey. 2014. Exposure of cerium oxide nanoparticles to kidney bean shows disturbance in the plant defense mechanisms. J. Hazard. Mat. 278: 279–287.
Mandeh, M., M. Omidi, and M. Rahaie. 2012. In vitro influences of TiO2 nanoparticles on barley (Hordeumvulgare L.) tissue culture. Biol. Trace Elem. Res. 150: 376–380.
Mattiello, A., P. Filip, F. Guido, Z. Costanza, F. Marta, P. Barbara, V. Massimo, M. Fabiano, M. Rita, and M. Luca. 2016. Engineered nanomaterials and crops: physiology and growth of barley as affected by nanoscale cerium oxide. Italian J. Agron. 11: 725.
Michel, B.E., and M.R. Kaufman. 1983. The osmotic potential of polyethylene glycol 6000. Plant Physiol. 51: 914–916.
Mojab, M., Gh.R. Zamani, S.V. Eslami, M. Hosini and S.A. Naseri. 2010. Study the Effect of Salinity and Drought Stresses Due to NaCl and PEG on Germination Characteristics and Seedling Growth of Jungle rice (Echinochloa crus-galli Var:oryzicola). J. Plant Protection. 24: 108-114. (In Persian, with English Abstract)
Moradi, A., F. Sharif Zadeh, R. Tavakkol Afshari, and R. Maali Amiri. 2015. Increasing seed germination efficiency of tall wheat grass (Agropyron elongatum (Host.) P. Beauv) at low temperature and drought stress conditions using urea osmopriming. Iran. J. Seed Res. 1:19–31. (In Persian, with English Abstract)
Muscolo, A., M. Sidari, U. Anastasi, C. Santonoceto, and A. Maggio. 2014. Effect of PEG-induced drought stress on seed germination of four lentil genotypes. J. Plant Interact. 9: 354–363.
Nakano, Y., and K. Asada. 1987. Purification of ascorbate peroxidase in spinach chloroplast: inactivation in ascorbate-depleted medium and reactivation by monodehydro ascorbate radical. Plant Cell Physiol. 28: 131–140.
Piccinno, F., F. Gottschalk, S. Seeger, and B. Nowack. 2012. Industrial production quantities and uses of ten engineered nanomaterials for Europe and the world. J. Nanopart. Res. 14: 1109–1120.
Qu, C., C. Liu, F. Guo, C. Hu, Y. Ze, C. Li, Q. Zhou, and F. Hong. 2013. Improvement of cerium on photosynthesis of maize seedlings under a combination of potassium deficiency and salt stress. Biol. Trace Elem. Res. 155: 104–113.
Rahemi Karizaki, A., M. Taji, N. Rasaei, and A. Jamali. 2015. Study the allelopathtic effect of the Harmala (Peganum harmala) on seed germination and seedling growth characteristics of new wheat cultivar of Gonbad compared to common wheat cultivar in Golestan Province. Iran. J. SeedSci. Technol. 4: 41–49. (In Persian, with English Abstract)
Rico, C.M., J. Hong, M.I. Morales, L. Zhao, A.C. Barrios, J.Y. Zhang, J.R. Peralta-Videa, and J.L. Gardea-Torresdey. 2013. Effect of cerium oxide nanoparticles on rice: a study involving the antioxidant defense system and in vivo fluorescence imaging. Environ. Sci. Technol. 47: 5635−5642.
Rico, C.M., J.R. Peralta-Videa, and J.L. Gardea-Torresdey. 2015. Differential effects of cerium oxide nanoparticles on rice, wheat, and barley roots: A Fourier Transform Infrared (FT-IR) microspectroscopy study. Appl. Spectrosc. 69: 287-295.
Rico, C.M., S.C. Lee, R. Rubenecia, A. Mukherjee, J. Hong, J.R. Peralta-Videa, and J.L. Gardea-Torresdey. 2014. Cerium oxide nanoparticles impact yield and modify nutritional parameters in wheat (Triticum aestivum L.). J. Agric. Food Chem. 62: 9669–9675.
Rossi, L., W. Zhang, L. Lombardini, and X. Ma. 2016. The impact of cerium oxide nanoparticles on the salt stress responses of Brassica napus L. Environ. Pollut. 219: 28–36.
Saeidi, M., M. Abdoli, M. Azhand, and S. Jalali-Honarmand. 2014. The effect of water deficiency stress and foliar application of indole acetic acid at different stages of grain growth on grain yield and germination traits of produced seeds in bread wheat cultivars.  Iran. J. SeedSci. Technol. 3: 173-187. (In Persian, with English Abstract)
Sajedi, N., H. Madani, and A. Sajedi. 2016. The effects of seed priming using distillate water and different rates of selenium on germination, seedling growth traits and seed yield of dryland wheat in laboratory and field condition. Iran. J. SeedSci. Technol. 5: 1–14. (In Persian, with English Abstract)
Sauret-Güeto, S., G. Calder, and N.P. Harberd. 2012. Transient gibberellin application promotes Arabidopsis thaliana hypocotyl cell elongation without maintaining transverse orientation of microtubules on the outer tangential wall of epidermal cells. Plant J. 69: 628–639.
Sharma, P., A.B. Jha, R.S. Dubey, and M. Pessarakli. 2012. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J. Bot. 2012: 1-26.
Siddiqui, M.H., M.H. Al-Whaibi, and M.O. Basalah. 2011. Role of nitric oxide in tolerance of plants to abiotic stress. Protoplasma. 248: 447–455.
Siddiqui, M.H., S.A. Alamri., M.Y.Y. Al-Khaishany, M.A. Al-Qutami, H.M. Ali, and Nasir Khan M. 2017. Sodium nitroprusside and indole acetic acid improve the tolerance of tomato plants to heat stress by protecting against DNA damage, J. Plant Interact. 12: 177-186.
Tian, X., and Y. Lei. 2006. Nitric oxide treatment alleviates drought stress in wheat seedlings. Biol. Plant. 50: 775–778.
Trujillo-Reyes, J., S. Majumdar, C. Botez, J. Peralta-Videa, and J. Gardea-Torresdey. 2014. Exposure studies of core–shell Fe/Fe3O4 and Cu/CuO NPs to lettuce (Lactuca sativa) plants: are they a potential physiological and nutritional hazard? J. Hazard. Mater. 267: 255–263.
Tumburu, L., C.P. Andersen, P.T. Rygiewicz, and J.R. Reichman. 2015. Phenotypic and genomic responses to titanium dioxide and cerium oxide nanoparticles in Arabidopsis germinants. Environ. Toxicol. Chem. 34: 70–83.
Vashisth, A., and S. Nagarajan. 2010. Effect on germination and early growth characteristics in sunflower (Helianthus annuus) seeds exposed to static magnetic field. J. Plant. Physiol. 167: 149–156
Wu, M., F. Wang, C. Zhang, Y. Xie, B. Han, J. Huang, and W. Shen. 2013. Heme oxygenase-1 is involved in nitric oxide- and cGMP-induced α-Amy2/54 gene expression in GA-treated wheat aleurone layers. Plant Mol. Biol. 81: 27–40.
Zangi, R., and M. Filella. 2012. Transport routes of metalloids into and out of the cell: a review of the current knowledge. Chem. Biol. Interact. 197: 47–57.
Zhang, W., S.D. Ebbs, C. Musante, J.C. White, C. Gao, and X. Ma. 2015. Uptake and accumulation of bulk and nanosized cerium oxide particles and ionic cerium by radish (Raphanus sativus L.). J. Agric. Food Chem. 63: 382–90.
Zhao, L., B. Peng, J.A. Hernandez-Viezcas, C. Rico, Y. Sun, J.R. Peralta-Videa, X. Tang, G. Niu, X. Jin, A. Varela-Ramirez, J.Y. Zhang, and J.L. Gardea-Torresdey. 2012. Stress response and tolerance of Zea mays to CeO2 nanoparticles: cross talk among H2O2, heat shock protein and lipid peroxidation. ACS Nano. 6: 9615–9622.
Zheng, C.F., D. Jiang, F. Liu, T.B. Dai, W.H. Liu, Q. Jing, and W.X. Cao. 2009. Exogenous nitric oxide improves seeds germination in wheat against mitochondrial oxidative damage induced by high salinity. Environ. Exp. Bot. 67: 222–227.