بررسی اثر پرایمینگ بر میزان فعالیت آنزیم های آنتی اکسیدانت بذر ذرت هیبرید سینگل کراس 704 تحت تنش خشکی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد علوم و تکنولوژی بذر/گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.

2 دانشیار/ گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.

چکیده

در پژوهش حاضر اثر انواع پرایمینگ برمیزان فعالیت آنزیم­های آنتی اکسیدانت شامل کاتالاز (CAT)،آسکوربات­پراکسیداز (APX)، سوپراکسید دیسمیوتاز (SOD)، مالون دی­آلدئید (MDA) و گلوتاتیون ردکتاز (Gred) تحت تنش خشکی برای بذور ذرت هیبرید سینگل کراس 704  بررسی شد. تیمارهای آزمایش، شامل تیمارهای خشکی در 4 سطح (0، 3-، 6-و 9- بار) و پرایمینگ (هورمون پرایمینگ، هیدروپرایمینگ، اسموپرایمینگ، اسید آسکوربیک و شاهد) بودند. سپس صفات درصد جوانه ­زنی، بنیه گیاهچه و میزان فعالیت آنزیم­های آنتی اکسیدانت اندازه­ گیری شد. نتایج نشان داد که اثر متقابل تیمار خشکی و پرایمینگ بر تمامی صفات به جز درصد جوانه ­زنی معنی ­دار بود. بیشترین بنیه گیاهچه (15.996) در تیمار پرایمینگ شاهد و سطح خشکی شاهد به دست آمد. بیشترین میزان فعالیت آنزیم کاتالاز (35 میکرومول بر دقیقه در میلی گرم پروتئین) در پرایمینگ با اسیدآسکوربیک و سطح خشکی 9- باردیده شد. همچنین حداکثر میزان آسکوربات پراکسیدازدر تیمار هورمون پرایمینگ و سطح خشکی 9- بار به دست آمد در حالی که کم­ترین فعالیت آن در سطح خشکی شاهد و تیمار اسموپرایمینگ مشاهده شد.بیشترین میزان مالون دی آلدئید در سطح خشکی 9- بار و در پرایمینگ شاهد به دست آمد. بالاترین میزان فعالیت آنزیم سوپراکسید دیسمیوتازدر سطح خشکی 9- بار بود. همچنین بیشترین میزان گلوتاتیون ردکتاز در پرایمینگ با ویتامین c و سطح خشکی 9- بار به دست آمد.

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