نقش باکتری‌های محرک رشد گیاهی بر برخی صفات جوانه‌زنی و ظهور گیاهچه در آزمایشگاه، صفات مورفولوژیکی و میزان رنگدانه‌های یونجه اکوتیپ همدانی(Medicago sativa L.) در شرایط گلخانه

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشکده کشاورزی دانشگاه شهرکرد

2 دانشکده کشاورزی، دانشگاه شهرکرد

3 دانشکده علوم پایه- دانشگاه شهرکرد

4 دانشکده کشاورزی - دانشگاه شهرکرد

چکیده

به منظور مقایسه تاثیر باکتری‌های محرک رشد بر جوانه‌زنی بذر یونجه همدانی آزمایشی در قالب طرح بلوک کامل تصادفی با 4 تکرار و 8 تیمار تلقیح باکتریایی (شامل عدم تلقیح، کاربرد باکتریAcinetrobacter calcoaceticus PTCC 1318،Bacillus megaterium PTCC 1250، Enterobacter aerogenes PTCC 1221 بصورت منفرد و کاربرد توام دوگانه و سه گانه آن‌ها) انجام گرفت. آزمایش دیگری در گلخانه بصورت فاکتوریل در قالب طرح کاملا تصادفی با 3 تکرار انجام شد. تیمارهای باکتریایی ذکر شده بعنوان عامل اول و تزریق و عدم تزریق باکتری به خاک گلدان‌ها بعنوان عامل دوم در نظر گرفته شدند. نتایج نشان داد کاربرد باکتری شاخص وزنی بنیه گیاهچه، درصد و سرعت جوانه‌زنی را افزایش داد اما بر دیگر صفات یونجه در آزمایشگاه اثری نداشت. تلقیح بذرها با باکتری در گلخانه اثرات مثبت بر رشد گیاه یونجه گذاشت و درصد و سرعت سبز شدن، وزن خشک گیاه، حجم و طول ریشه، میزان کلروفیل a، b و کلروفیل کل برگ را افزایش داد. تزریق باکتری پای گیاهچه نیز برخی صفات را تحت تاثیر قرار داد که می‌تواند به دلیل استقرار بهتر و جمعیت بیشتر باکتری در ریزوسفر باشد. در مجموع کاربرد منفرد باکتری نتایج بهتری نسبت به کاربرد دو و سه باکتری با هم داشت لذا به نظر می‌رسد اثرات ضدیت باکتری‌ها بر یکدیگر ممکن است اثرات مثبت آن‌ها را کاهش دهد.

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