ارزیابی تأثیر تغییرات بیوشیمیایی ایجاد شده در مدت هیدروپرایمینگ بر بنیه بذر سه رقم گندم

نوع مقاله: مقاله پژوهشی

نویسندگان

دانشگاه شهید چمران اهواز

چکیده

در آزمایش اول، اثر 7 مدت زمان خیساندن بذر (0 ، 6 ، 8 ، 10 ، 12 ، 14 و 16 ساعت ) بر ویژگیهای جوانه زنی و رشد گیاهچه ارقام گندم استار، چمران و فونگ مورد بررسی قرار گرفت. در آزمایش دوم، برخی صفات بیوشیمیایی و فعالیت های آنزیمی در بذرهای پرایم شده در سه مدت زمان 6 ، 8 و 10 ساعت اندازه گیری شد. نتایج آزمایش اول نشان داد که هیدروپرایمینگ با مدت 8 ساعت خیساندن بیشترین اثرات مثبت را بر بنیه بذر ارقام گندم داشت. درمیان ارقام نیز سرعت جوانه زنی در رقم های استار و چمران با یکدیگر اختلاف معنی داری نداشت و کمترین سرعت جوانه زنی در رقم فونگ مشاهده شد. بیشترین طول و وزن خشک ریشه چه در رقم استار مشاهده شد. نتایج آزمایش دوم نشان داد که با افزایش مدت هیدرو پرایمینگ، فعالیت آنزیم α آمیلاز و محتوی قندها و پروتئین های محلول در بذر افزایش یافت. همچنین افزایش محتوی مالون دی آلدئید و فعالیت ناکافی آنزیم های آنتی اکسیدان (افزوده نشدن فعالیت آنزیم با افزایش مدت زمان هیدروپرایمینگ یا کاهش فعالیت آنزیمی) در مدت زمان 10 ساعت هیدروپرایمینگ نسبت به مدت زمان 8 ساعت مشاهده گردید. درمیان ارقام نیز بیشترین و کمترین فعالیت آنزیم α آمیلاز ، محتوی قندها و پروتئین های محلول به ترتیب متعلق به رقم های استار و فونگ بود. در این آزمایش همبستگی فعالیت آنزیم های آنتی اکسیدان با ویژگیهای جوانه زنی و رشد گیاهچه مثبت و معنی دار بود.

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