تأثیر سویه های مختلف باکتری سودوموناس فلورسنس(Pseudomona fluorescence) بر جوانه زنی و رشد گیاهچه دو رقم جو

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار دانشگاه گیلان، دانشکده کشاورزی، گروه زراعت و اصلاح نباتات

2 کارشناسی ارشد دانشگاه گیلان، دانشکده کشاورزی، گروه زراعت و اصلاح نباتات

چکیده

به منظور بررسی تأثیر سویه های مختلف باکتری سودوموناس فلورسنس بر جوانه زنی و رشد گیاهچه جو رقم فصیح و بهمن، آزمایشی در دانشکده کشاورزی دانشگاه گیلان به صورت فاکتوریل و در قالب طرح کاملاً تصادفی با 3 تکرار به اجرا درآمد. در این آزمایش بذرها با سویه های 36، 103، 93، 36+93، 93+103، 103+36 و 36+93+103 سودوموناس فلورسنس تلقیح شدند و تیمار بدون تلقیح با باکتری به عنوان شاهد در نظر گرفته شد. صفات مورد بررسی شامل وزن تر و خشک ساقه چه، وزن تر و خشک ریشه چه، طول ساقه چه و ریشه چه، سرعت و درصد جوانه زنی، شاخص بنیه و درصد آب گیاهچه بودند. نتایج به دست آمده نشان داد که اثر باکتری بر همه عوامل معنی دار بود، به طوری که تلفیق سه باکتری 103+ 63 + 93 سودوموناس فلورسنس بیشترین اثر را بر عوامل مورد بررسی نسبت به کاربرد آن ها به صورت تنهایی و دوتائی، نشان دادند. برهمکنش باکتری در رقم به غیر از وزن تر و خشک ریشه چه و طول ریشــه چه بر صفـات دیگر معنی دار بود. در بین ارقام مورد مطالعه نیز رقم فصیح تنها از نظر شاخص بنیه، سرعت جوانه زنی و وزن تر ساقه چه بر رقم بهمن برتری نشان داد و در سایر صفات، رقم بهمن بهتر بود.

کلیدواژه‌ها


Abdual-baki, A. A. and J. D. Anderson. 1973. Relationship between decarboxilation of glutamic acid and
vigour in soybean seed. Crop Sci, 13: 222-226. Ahmad, F., L. Ahmad and M. S. Khan. 2006. Screening of free-living rhizospheric bacteria for their
multiple plant growth promoting activities. Microbial. Res.,Vol. 36, pp: 1-9. 1 
Akazawa, T. and I. Hara-Mishimura. 1985. Topographic aspects of biosynthesis, extracellular section and
intracellular storage of proteins in plant cells. Ann. Rev. Plant Physiol. 70: 441-472. Akhgar, A., K. Khavazi and N. Khakipoor. 2011. Isolation, identification and effectiveness of ACC- deaminase producing rhizobacteria on the alleviation of salinity stress effects on canola growth. J. Water
Soil. Vol. 25, pp: 29-41. Alef, K. and P. Nannipieri. 1995. Methods in Applied Soil Microbiology and Biochemistry. Academic
Press. Bayari, A., S. Nezarat and A. Gholami. 2009. Relationship between germination index of seed corn with
inoculation of PGPR (Pseudomonas, Azospirillium and Azotobacter).11
th Soil Science Congress of Iran, Gorgan. Beck, E. and P. Ziegler. 1989. Biosynthesis and degradation of starch in higher plants. Annu Rev. Plant
Physiol. Plant Mol. Biol., 40: 95-117. Becking, J. H. 2006. Prokaryotes 6: 759-783. Belimov, A. A., V. I. Safronova, T. A. Sergeyeva, T. N. Egorova, V. A. Matveyeva, V. E. Tsyganov, A. Y. Borisov, I. A. Tikhonovin, C. Kluge, A. Preisfeld, K. J. Deitz and V. V. Stepanok. 2001. Characterization of plant growth promoting rhizobacteria isolated from polluted soils and containing 1- aminocyclopropane-1-carboxylate deaminase. Can. J. Microbiol. 47: 642-652. Bharathi, R., R. Vivekananthan, S. Harish, A. Ramanathan and R. Samiyappan. 2004. Rhizobacteria- based bio-formulations for the management of fruit rot infection in chillies. Crop Protec. 23: 835ñ843. Cartieaux, F. P., L. Nussaume and C. Robaglia. 2003. Tales from the underground: molecular plant
rhizobacteria interactions. Plant, Cell Environ, 26: 189-199. Cassan, F., D. Perriga, V. Sgroya, O. Masciarellia, C. Pennab, V. Lunaa. 2009. Azospirillum brasilense
Az39 and Bradyrhizobium japonicum E109, inoculated singly or in combination, promote seed germination
and early seedling growth in corn (Zea mays L.) and soybean (Glycine max L.). Eur. J. Soil Biol, 45, 28ñ35. Cattelan, A. J., P. G. Hartel and J. J. Fuhrmann. 1999. Screening for plant growth-promoting
rhizobacteria to promote early soybean growth. Soil Sci. Soc. Am. J., Vol. 63, pp: 1670ñ1680. Dileep Kumar, S. B., I. Berggren and A. M. Martensson. 2001. Potential for improving pea production by
coinoculation with Fluorescent Pseudomonas and Rhizobium. Plant Soil, 229: 25-34. Egamberdiyeva, D. 2007. The effect of plant growth promoting bacteria on growth and nutrient uptake of
maize in two different soils. Appl. Soil. Eco. 36: 184-189. Ehteshami, S. M., M. Aghaalikhani, M. R. Chaichi and K. Khavazi. 2009. The effect of phosphorus
biological fertilizer on quality and quantity corn on water tolerance. Iranian J. Field Crop Sci, 40: 15-27. Ellis, R. H. and E. H. Roberts. 1980. Towards a rational basis for testing seed quality in seed production. 605-635. Butterworths. London. Gholami, A., S. Shahsavani and S. Nezarat. 2009. The Effect of plant growth promoting rhizobacteria
(PGPR) on germination, seedling growth and yield of maize. World Aca. Scie, Engineering and Technol, p:
19-24. Goldstein, A. 1986. Bacterial solubilizatin of mineral phosphates. Am. J. Altern.Agri., 1:51-57. Hampton, J. G. and D. M. Tekrony. 1995. Handbook of vigour test methods. International Seed Testing
Association (ISTA). Zurich, Swirztland, 730p. Hernandez, A. N., A. Hernandez and M. Heydrich. 1995. Selection of rhizobacteria for use in maize
cultivation. Cultivos Tropicales, 6: 5-8. Kaymak, H. A., I. Guvenc, F. Yarali and M. F. Denmez. 2009. The effects of bio-priming with PGPR on
germination of radish (Raphanus sativus L.) seeds under saline conditions. Turk. J. Agric, 33: 173-179. Kiers, E. T. and R. F. Denison. 2008. Sanctions, cooperation, and the stability of plantrhizosphere
mutualisms. Ann. Rev. Ecol. Environ. System 39: 215-236. Klee, H. J., M. B. Hayford, K. A. Kretzmer, G. F. Barry and G. M. Krishore. 1991. Control of ethylene
sysnthesis by expression of a bacterial enzyme in transgenic tomato plants. Plant Cell 3:1187-1193. Kundu, B. S. and A. C. Gaur. 1980. Establishment of nitrogen fixing and phosphate solubilizing bacteria in
rhizosphere and their effect on yield and nutrient uptake of wheat crop. Plant Soil. 57: 223ñ230. Lucy, M., E. Reed and B. R. Glick. 2004. Applications of free living plant growth promoting rhizobacteria. Antonie van Leeuwenhoek, 86: 1ñ25. Lugtenberg, B., T. Chin-A-Woeng and G. Bloemberg. 2002. Microbeñplant interactions: principles and
mechanisms. Antonie van Leeuwenhoek. 81: 373ñ383. Mantelin, S. and B. Touraine. 2004. Plant growth promoting bacteria and nitrate availability: impacts on
root development and nitrate uptake. J. Expt. Bot. 55: 27-34. Mayak, S. 2004. Plant growth promoting bacteria confer resistance in tomato plants to salt stress. Plant
Physiol. Biochem. 42: 565-572.
 
Mia, M. A. B., Z. H. Shamsuddin, W. Zakaria and M. Mariah. 2009. The effect of rhizobacterial
inoculation on growth and nutrient accumulation of tissue-cultured banana plantlets under low N-fertilizer
regime. Afr. J. Biotechnol. 8: 5855-5866. Mishra, M., U. Kumar, P. K. Mishra and V. Prakash. 2010. Efficiency of Plant Growth Promoting
Rhizobacteria for the Enhancement of Cicer arietinum L. Growth and Germination under Salinity. Adv. Biol. Res. 4 : 92-96. Misko, A. L. and J. J. Germida. 2002. Taxonomic and functional diversity of Pseudomonas isolated from
the roots of field-grown canola. FEMS Microbiol. Ecol. 42: 399ñ407. Ng, L. C., M. Sariah, O. Sariam, O. Radziah, M. A. Zainal Abidin. 2012. Rice seed bacterization for
promoting germination and seedling growth under aerobic cultivation system. Aus. J. Crop Sci, 6(1): 170- 175. Noumavo, P. A., E. Kochoni, Y. O. Didagbe, A. Adjanohoun, M. Allagbe, R. Sikirou, E. W. Gachomo, S. O. Kotchoni, L. Baba-Moussa. 2013. Effect of Different Plant Growth Promoting Rhizobacteria on
Maize Seed Germination and Seedling Development. Ame. J. Plant Scie. 4: 1013-1021. Patten, C. and B. R. Glick. 1996. Bacterial biosynthesis of indole-3-acetic acid. Cana. J. Microbiol. 42: 207- 220. Pieta-Filho, C. and R. H. Ellis. 1991. The development of seed quality in spring barley in four
environments: A. Germination and longevity. Seed Science Research, 1: 163-177. Saleem , M., M. Arshad, S. Hussain and A. S. Bhatti. 2007. Perspective of plant growth promoting
rhizobacteria (PGPR) containing ACC deaminase in stress agriculture. J. Ind. Microbiol. Biotechnol. 34:
635ñ648. Scott, S. J., R. A. Jones and W. A. Williams. 1984. Review of data analysis methods for seed germination. Crop Scie. 24: 1192-1199. Shaharoona, B., M. Arshad, Z. A. Zahir and A. Khalid. 2006. Performance of Pseudomonas spp. containing ACC-deaminase for improving growth and yield of maize (Zea mays L.) in the presence of
nitrogenous fertilizer. Soil. Biol. Biochem.. 38: 2971ñ2975. Shanmugaiah, V., N. Balasubramanian, S. Gomathinayagam, P. T. Manoharan and A. Rajendran. 2009. Effect of single application of Trichoderma viride and Pseudomonas fluorescens on growth promotion
in cotton plants. African Journal of Agri. Res. 4: 1220-1225. Sharma, K., G. Dak, A. Agrawal, M. Bhatnagar and R. Sharma. 2007. Effect of phosphate solubilizing
bacteria on the germination of Cicer arietinum seedes and seedling growth. J. Herbal Med. Toxicol. pp:59- 61. Shaukat, K., S. Affrasayab and S. Hasnain. 2006. Growth responses of Helianthus annus to plant growth
promoting rhizobacteria used as a biofertilizer. J. Agri. Res. 1: 573-581. Tsonev, T. D., G. N. Lazova, Z. G. Stoinova and L. P. Popova. 1998. A possible role for Jasmonic acid in
adaptation of barley seedlings to salinity stress. Plant Growth Regu. 17: 153-159. Verma, J. P., J. Yadav and K. N. Tiwari. 2010. Application of Rhizobium sp. BHURC01 and plant growth
promoting rhizobactria on nodulation, plant biomass and yields of chickpea (Cicer arietinum L.). Int. J. Agric. Res. 5: 148-156. Walley, F. L. and J. J. Germida. 1997. Response of spring wheat (Triticum aestivum L.) to interactions
between Pseudomonas species and Glomus clarum NT4. Biol. Fertility Soils. 24: 365ñ371. Yadav, J., J. P. Verma and K. N. Tiwari. 2010. Effect of Plant Growth Promoting Rhizobacteria on Seed
Germination and Plant Growth Chickpea (Cicer arietinum L.) under in Vitro Conditions. Biol. Forum-Ann. Int. J. 2: pp. 15-18. Zaidi, S. F. A. 2003. Inoculation with Bradyrhizobium japonicum and fluorescent Pseudomonas to control
Rhizoctonia solani in soybean [Glycine max (L) Merr]. Ann. Agric Res. 24: 151-153.