تاثیر تلقیح بذر با باکتری های آزادزی تثبیت کننده نیتروژن و زمان محلول پاشی کود نیتروژنه بر مشارکت ذخایر ساقه در عملکرد دانه، سرعت و طول دوره موثر پر شدن دانه تریتیکاله

نوع مقاله: مقاله پژوهشی

نویسندگان

1 گروه زراعت و اصلاح نباتات دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی

2 گروه علوم دامی دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی

چکیده

 به منظور بررسی تاثیر کاربرد باکتری های آزاد زی تثبیت کننده نیتروژن و زمان محلول­پاشی کود نیتروژنه بر میزان مشارکت ذخایر ساقه در عملکرد دانه، سرعت و طول دوره پر شدن دانه تریتیکاله، آزمایشی به صورت فاکتوریل در قالب طرح بلوک­های کامل تصادفی در سه تکرار در مزرعه تحقیقاتی دانشکده کشاورزی دانشگاه محقق اردبیلی در سال 1391 انجام گردید. تیمار­ها شامل زمان محلول­پاشی کود نیتروژنه در چهار سطح (عدم محلول­پاشی، محلول­پاشی در زمان چکمه پوش، ظهور سنبله و دوره پرشدن دانه) و تلقیح بذر با باکتری­های محرک رشد در چهار سطح (عدم تلقیح بذر با باکتری­های محرک رشد، تلقیح بذر با ازتوباکتر کروکوکوم استرین 5، آزوسپریلوم لیپوفروم استرین OF و سودوموناس پوتیدا استرین 9) بود. نتایج نشان داد عملکرد و اجزای عملکرد دانه، سرعت و طول دوره موثر پرشدن دانه به طور معنی­داری تحت تاثیر تلقیح بذر با باکتری های آزادزی تثبیت کننده و محلول­پاشی کود نیتروژنه قرار گرفت. حداکثر وزن دانه (0/054 گرم) و طول دوره موثر پرشدن دانه (34/17 روز) از محلول­پاشی در زمان چکمه پوش در تلقیح بذر با ازتوباکتر بدست آمد. بیشترین مشارکت ذخایر ساقه در عملکرد دانه (30/63 درصد) در حالت عدم تلقیح بذر با باکتری­های محرک رشد و عدم محلول­پاشی کود نیتروژنه و کمترین آن (8/12 و 8/13 درصد) از محلول­پاشی در زمان چکمه پوش در تلقیح بذر با ازتوباکتر و محلول پاشی در مرحله ظهور سنبله در تلقیح بذر با ازتوباکتر بدست آمد. به نظر می رسد که به منظور افزایش عملکرد، سرعت و طول دوره موثر پرشدن دانه می توان پیشنهاد کرد که تلقیح بذر با ازتوباکتر و محلول پاشی با کود نیتروژنه در زمان چکمه پوش به کار برده شود.

کلیدواژه‌ها


Barnett, K.H., and P.B. Pearce.1983. Source-Sink ratio alteration and its effect on physiological  parameters in maize. Crop Sci. 23: 294-299.

Cakmakci, R.I., M.F. Donmez., and U. Erdogan. 2007. The effect of plant growth promoting rhizobacteria on barely seedling growth, nutrient uptake, some soil properties, and bacterial counts. Turkish  J  of  Agric. 31: 189-199.

Cho, D.S., S.K. Jong., and Y.K. Park. 1987. Studies on the duration and rate of drain filling in rice (Oryza sativa L.). I. Varietal difference and effects of nitrogen. Korean J of  Crop Sci. 23: 103-111.

Dart, P.J., and  J.M. Day. 1975. Nitrogen fixation in the field other than by nodules. In: N. Walker (Ed), Soil Microbiol. Butter Worth  Sci. Publication, London. pp: 112-119.                                                          

Dobereiner, J., J.M. Day., and P.J. Dart. 1972. Nitrogenase activity and oxygen sensivity of the Paspalum notatum-Azotobacter paspali association. J. of General Microbiol. 71: 103-116.                                          

Ehdaie, B., and J.G. Wanies. 1996. Genetic variation for contribution of preanthesis assimilates to grain yield in spring wheat. J.  of  Genet. and Breed. 50: 47-56.

Ellis, R.H., and C. Pieta-Filho. 1992. The development of seed quality in spring and winter cultivars of barely and wheat. Seed  Sci. 2: 19-25.

Feiziasl,V., and GH.R.Valizadeh. 2004. Effect of urea liquid fertilizer spraying at different plant growth stages on grain quality and quantity in Sardari dryland wheat (T. aestivum. L.). Iranian J. of Agric. Sci. 35:2 (In Persian, with English Abstract)

Garangac, A.G.,  and S. Galeshi. 2000. Effects of foliar application of urea on yield and yield components of two cultivars wheat (Triticum aestivum L.). Agric. Sci. and Nat. Res. 8 (2): 87-97. (In Persian, with English Abstract)

Gooding, M.I., and W.P. Davies. 1992. Foliar urea fertilization of cereals. Fert. Res. 32: 202-222.

Hamdi,Y.A. 2002. Application of nitrogen fixing systems in soil improvement and management. FAO Soil Bull, Rome, 188p.                                                                                                                           

 Jagnow, G. 1987. Inoculation of cereal crops and forage grasses with nitrogen-fixing rhizosphere bacteria: possible causes of success and failure with regard to yield response-a review. Agron.  J. 15: 361-368.

James, E.H. and G.M. Paulsen. 2004. Nitrogen assimilation and protein synthesis in wheat seedlings as affected by mineral  nutrition. Plant  Physiol. 44(5): 636-640.

Kandil, A.A., M.A. Badawi, S.A. EL-Moursy. and M.A. Abdou. 2004. Effect of planting dates, nitrogen levels and bio- fertilization treatments on 1: Growth attributes of sugar beet (Beta Vulgaris L.). Basic Appl Sci. 5(2): 227-237.                                                                                                                    

Kaya,Y.K., R.Z. Arisoy., and A. Gocmen. 2002. Variation in grain yield and quality traits of bread wheat genotypes by zinc fertilization. Pakistan  J. of  Bot. 1(4): 142-144.

Kennedy, I.R., A.T. Choudhury., and M.L. Kecskes. 2004. Non-symbiotic bacterial diazotrophs in crop-farming systems: can their potential for plant growth promotion be better exploited? Soil  Biol. and  Biochem.36:1229-1244.                                                                                                                               

KhoshGoftar Manesh, A. 2008. Princple of Plant Nutrition. University of Esfahan Press. (In Persian).

Kloepper, J.W., and C.J. Beauchamp.1992.A review of issues related to measuring of plant roots by bacteria. Can.  J. of  Microbiol. 38: 1219-1232.

Manske, G.B., A. Luttger., R.K. Behi., P.G. Vlek. and M. Cimmit. 2000. Enhancement of mycorhiza (VAM) infection, nutrient efficiency and plant growth by Azotobacterchroococcum in wheat. Plant  Breed. 13: 78-83.

Masoni, A., L. Ercoli., M. Mariotti., and I. Arduini. 2007. Post-anthesis accumulation and remobilization of dry matter, nitrogen and phosphorus in drum wheat as affected by soil type. Eur  J  of Agron. 26: 179-186.

Mishra. M., A.K. Patjoshi., and D. Jena. 1998. Effect of biofertilization on production of maize (Zea mays). Indian  J of Agron 43: 307–310.

Murchie, E.H., J. Yang., S. Hubbart., P. Horton., and S. Peng. 2002. Are there associations between grain-filling rate and photosynthesis in the flag leaves of field grown rice? J. of  Eur.  Sci. 53: 2217-2224.

Ntanos, D.A., and S.D. Koutroubas. 2002.Dry matter and N accumulation and  translocation for India and Japonica rice under Mediterranean conditions. Field Crops Res.74: 93-101.

Papakosta, D.K., and A.A. Gagianas. 1991.Nitrogen and dry matter accumulation, remobilization, and losses for Mediterranean wheat during grain filling. Agron J. 83: 864-870.

Peltonen, J. 1993. Interaction of late season foliar spray of urea and fungicide mixture in wheat production. J of  Agron and  Crop Sci. 170: 296-308.                                                                                            

Rao, S.C., and T.H. Dao.1992. Fertilizer placement and tillage effects of nitrogen assimilation by wheat. Agron.  J. 84:1028- 1032. 

Ronanini, D., R. Savin.,and AJ. Hall.2004. Dynamic of fruit growth and oil quality of sunflower (Helianthus annuus L.) exposed to brief interval of high temperature during grain filling. Field  Crop Res.  83: 79-90.

Rudresha, D.L., M.K. Shivaprakasha., and R.D. Prasad.2005. Effect of combined application of Rhizobium, phosphate solubilizing bacterium and Trichoderma spp. on growth, nutrient uptake and yield of chickpea (Cicer aritenium L.). Appl. Soil and Ecol. 28:139–146.

Salwau, M.I.M. 1994. Effect of soil and  foliar application of nitrogen levels on yield and yield components of wheat (T. aestivum L.).Annals Agric Sci: 32: 705-715.                                

Saradan, S.J., and M.C. Gianibelli.1990. Effect of foliar urea spraying and nitrogen application at sowing upon dry matter and nitrogen distribution in wheat (Triticum aestivum L.). Agron.  J. 10: 183-189.         

Seyed Sharifi1, R., and H. Nazarly. 2012. Effects of seed priming with Plant Growth Promoting Rhizobacteria (PGPR) on grain yield, fertilizer use efficiency and dry matter remobilization of sunflower (Helianthus annus L.) with various levels of nitrogen fertilizer. J. of  Sustain Agric. 3:27-45.

Sharaan, A.N., and F.S. El-Samie.1999.Response of wheat varietiers to some environmental influences.1. Effect of seeding rates and N fertilization levels on growth and yield of two wheat varieties (Triticum aestivum L.). Am. Agric. Sci. 44: 589-601.

Singh, R., R.K. Behl., K.P. Singh., P. Jain., and N. Narula.2004.Performance and gene effects for wheat yield under inoculation of arbuscular mycorrhiza fungi and Azotobacter chroococcum. Haryana Agricultural University. Hisar, Plant Soil Environ. 50(9): 409-415.                                                                    

Souza, S.R., E. Mariam., L.M. Stark. and M.S. Fernandes. 1998. Nitrogen remobilization during the reproductive period in two Brazilian rice varieties. J of Plant Nut. 21: 2049-2053.

Syverud, T.D, L.M. Walsh, E.S. Oplinger.,  and  K.A. Kelling 1980. Foliar  fertilization of soybean (Glycine max L.). Commun.  Soil Sci and  Plant Nut. 11:637-651

Togay, N. and Togay.2008.Effect of Rhizobium inoculation, sulfur and phosphorus application on yield, yield components and nutrient uptake in chick pea (Cicer  aretinum L.). African  J of  Biotech. 7:6. 776-782.

Tosi Kohal,P., M. Esfahani., B. Rabei., and M. Rabei.2012.Effect of Concentration and Time of Supplementary Nitrogen Fertilizer Application on Yield and NUE of Rapeseed (Brassica napus L.) as a Second Crop in Paddy Field. 42(2):387-396. (In Persian, with English Abstract)

Tsuno, Y., T. Yamaguchi., and J. Nakano.1994. Potential dry matter production and grain filling process of rice plant from the viewpoint of source-sink relationships and the role of root respiration in its relationship. Agron.  J.  47: 1-10.

Yamaguchi, T., Y. Tsuno., J. Nakano., and K. Miki. 1995. Influence of nitrogen content on grain weight at the early ripening stage and relationship between root respiration and leaf area per spikelet of rice plants. Agron  J. 33:251-258

Zabihi, H.R., G.R. Savaghebi, K. Khavazi., and A. Ganjali. 2008. Response of wheat growth and yield to application of plant growth promoting rhizobacteria at various levels of phosphorus fertilization. Iranian J. of Field Crops. 7(1): 41-51. (In Persian, with English Abstract)